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Cytokeratin 14 Antibody / CK14 / KRT14

Cytokeratin 14 Antibody / CK14 / KRT14

Catalog Number: orb749633

DispatchUsually dispatched within 5-10 working days
$ 764.00
Catalog Numberorb749633
CategoryAntibodies
DescriptionCytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Species/HostMouse
ClonalityMonoclonal
Clone NumberKRT14/532
Tested applicationsIHC-P
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG3, kappa
ImmunogenRecombinant full-length human KRT14 protein was used as the immunogen for the Keratin 14 antibody.
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Keratin 14 antibody is available for research use only.
UniProt IDP02533
StorageStore the Keratin 14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Keratin 14 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
  • Cytokeratin 14 Antibody / CK14 / KRT14 [orb248446]

    FACS,  IF,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 20 μg
  • Cytokeratin 14 Antibody / CK14 / KRT14 [orb385868]

    IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 20 μg
  • Cytokeratin 14 Antibody / CK14 / KRT14 [orb639775]

    FACS,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 20 μg
  • Cytokeratin 14 Antibody / CK14 / KRT14 [orb750237]

    IF,  IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 20 μg
  • Cytokeratin 14 Antibody / CK14 / KRT14 [orb1825082]

    IHC-P

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μg
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