Cytokeratin 14 Antibody / CK14 / KRT14

• Catalog Number: orb385868
• Category: Antibodies
• Description: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CTKN14-1
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG3
• Immunogen: Recombinant full-length human KRT14 protein was used as the immunogen for the Keratin 14 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Keratin 14 antibody is available for research use only.
• UniProt ID: P02533
• Storage: Store the Keratin 14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Keratin 14 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate with Keratin 14 antibody (clone CTKN14-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min. followed by cooling at RT for 20 min.

IHC testing of FFPE human cervix with Keratin 14 antibody (clone CTKN14-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min. followed by cooling at RT for 20 min.