Cytochrome c Antibody

• Catalog Number: orb1260421
• Category: Antibodies
• Description: Cytochrome c Antibody
• Target: CYCS
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
• UniProt ID: P99999
• MW: Observed: 14kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c,
• Note: For research use only

Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (orb1260421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).

Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (orb1260421) at dilution of 1:100. Blue: DAPI for nuclear staining.