CYP3A5 Antibody (C-term)

• Catalog Number: orb1928887
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human CYP3A5.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P20815
• MW: 57109 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Clone Number: RB15796
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450 HLp
• Note: For research use only
• NCBI: NP_001177413.1, NP_000768.1

Western blot analysis of lysate from SK-BR-3 cell line, using CYP3A5 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-CYP3A5 Antibody (C-term) in 293 cell line lysates (35 ug/lane). CYP3A5 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of CYP3A5 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).