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Catalog Number | orb1258123 |
---|---|
Category | Antibodies |
Description | CYP3A4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:1000IHC: 1:20 - 1:50IF: 1:20 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 80kDa |
Target | CYP3A4 |
UniProt ID | P08684 |
Storage | Store at 4°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, pH 7.3. |
Alternative names | CYP3A4, cytochrome P450, family 3, subfamily A, po Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IHC: 1:20 - 1:50IF: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CYP3A4 antibody (orb1258123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat ovary using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CYP3A4 antibody (orb1258123) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse liver using CYP3A4 antibody (orb1258123) at dilution of 1:100. Blue: DAPI for nuclear staining.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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