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CYP3A4 Antibody
SKU: orb1258123
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Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2). |
| Target | CYP3A4 |
| Molecular Weight | Observed: 80kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−CYP3A4, cytochrome P450, family 3, subfamily A, polypeptide 4, HGNC:2637, CP33, CP34, CYP3A, CYP3A3, HLP, NF-25, P450C3, P450PCN1, P450-III, steroid inducible, subfamily IIIA (niphedipine oxidase), polypeptide 3, subfamily IIIA, glucocorticoid-inducible P450, nifedipine oxidase
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ELISA, IF, IHC-P, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
50 μl, 100 μl
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Western blot analysis of extracts of various cell lines, using CYP3A4 antibody (orb1258123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat ovary using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CYP3A4 antibody (orb1258123) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse liver using CYP3A4 antibody (orb1258123) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
CYP3A4
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CYP3A4 Antibody (orb1258123)
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