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Catalog Number | orb769391 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CYP39A1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome P450 39A1. AA range:361-410 |
MW | 54 |
Tested applications | ELISA, IF, IHC-P, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-CYP39A1 antibody, anti-24-hydroxycholesterol Read more... |
Note | For research use only |
Immunofluorescence analysis of A549 cells, using Cytochrome P450 39A1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from LOVO and HUVEC cells, using Cytochrome P450 39A1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |