CYP26B1 Antibody

• Catalog Number: orb1247633
• Category: Antibodies
• Description: CYP26B1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SQARSEDKDGRFH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 85kDa band in human testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_063938.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Cerebellum shows staining of cell bodies in the Molecular Layer. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Small Intestine, Spleen and Testis. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CYP26B1
• UniProt ID: Q9NR63
• NCBI: NP_063938.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CYP26B1, cytochrome P450, family 26, subfamily B,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 85kDa band in human testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_063938.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Cerebellum shows staining of cell bodies in the Molecular Layer. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Small Intestine, Spleen and Testis. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247633 (4 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

orb1247633 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247633 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247633 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.