CYP24A1 Antibody

SKU: orb1249009

Description

CYP24A1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human and Rat Liver lysates (calculated MW of 51.2kDa according to NP_001122387.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 3.75ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RKYDIQATDN
• Target: CYP24A1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

CYP24A1, cytochrome P450, family 24, subfamily A, polypeptide 1, CP24, CYP24, MGC126273, MGC126274, P450-CC24, 1,25-@dihydroxyvitamin D3 24-hydroxylase, 24-OHase, cytochrome P450 family 24 subfamily A polypeptide 1, cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase), exo-mitochondrial protein, vitamin D 24-hydroxylase

orb1249009 (0.1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249009 (3.75 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CYP24A1

UniProt

Q07973

RefSeq (Protein):NP_001122387.1, NP_000773.2