CYP21A2 Antibody

• Catalog Number: orb1262883
• Category: Antibodies
• Description: CYP21A2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CYP21A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the Central region of human CYP21A2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: CYP21A2
• UniProt ID: P08686
• NCBI: P08686
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CYP21A2 Antibody in MDA-MB435 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CYP21A2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CYP21A2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).