CYP17A1 rabbit pAb

SKU: orb767823

Description

Rabbit polyclonal antibody to CYP17A1

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cytochrome P450 17A1. AA range:221-270
• Molecular Weight: 50
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CYP17A1 antibody, anti-CYP17 antibody, anti-S17AH antibody, anti-Steroid 17-alpha-hydroxylase/17 antibody, anti-20 lyase antibody, anti-CYPXVII antibody, anti-Cytochrome P450 17A1 antibody, anti-Cytochrome P450-C17 antibody, anti-Cytochrome P450c17 antibody, anti-Steroid 17-alpha-monooxygenase antibody

Immunofluorescence analysis of Hela cell. 1, CYP17A1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Western Blot analysis of 293 cells using CYP17A1 Polyclonal Antibody diluted at 1:2000.

Western blot analysis of lysates from Jurkat, A549, and HeLa cells, using Cytochrome P450 17A1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).