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CYP17A1 rabbit pAb

Catalog Number: orb767823

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb767823
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CYP17A1
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
ImmunogenThe antiserum was produced against synthesized peptide derived from human Cytochrome P450 17A1. AA range:221-270
MW50
Tested applicationsELISA, IF, IHC-P, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-CYP17A1 antibody, anti-CYP17 antibody, anti-S
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NoteFor research use only
CYP17A1 rabbit pAb

Immunofluorescence analysis of Hela cell. 1, CYP17A1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

CYP17A1 rabbit pAb

Western Blot analysis of 293 cells using CYP17A1 Polyclonal Antibody diluted at 1:2000.

CYP17A1 rabbit pAb

Western blot analysis of lysates from Jurkat, A549, and HeLa cells, using Cytochrome P450 17A1 Antibody. The lane on the right is blocked with the synthesized peptide.

CYP17A1 rabbit pAb

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

  • CYP17A1 Antibody (Center) [orb1928799]

    IF,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Cytochrome P450 17A1 Rabbit pAb, PerCP-Cy7 conjugated [orb2863099]

    IF

    Bovine, Canine, Equine, Porcine, Rat, Sheep

    Human, Mouse

    Rabbit

    Polyclonal

    PerCP/Cy7

    100 μl
  • Cytochrome P450 17A1 Rabbit pAb, Pacific Blue conjugated [orb2863100]

    IF

    Bovine, Canine, Equine, Porcine, Rat, Sheep

    Human, Mouse

    Rabbit

    Polyclonal

    Pacific Blue

    100 μl
  • Cytochrome P450 17A1 Rabbit pAb, BF700 conjugated [orb2863101]

    IF

    Bovine, Canine, Equine, Porcine, Rat, Sheep

    Human, Mouse

    Rabbit

    Polyclonal

    BF700

    100 μl
  • Cytochrome P450 17A1 Rabbit pAb, AP conjugated [orb2863102]

    IHC-Fr,  IHC-P

    Bovine, Canine, Equine, Porcine, Rat, Sheep

    Human, Mouse

    Rabbit

    Polyclonal

    AP

    100 μl