Cyclophilin B Recombinant Rabbit Monoclonal Antibody (Loading Control)

SKU: orb704516

Description

Cyclophilin B Recombinant Rabbit Monoclonal Antibody (Loading Control)

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 4A10
• Immunogen: Recombinant human Cyclophilin B C terminus
• Target: PPIB
• Molecular Weight: 20 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Cphn 2; Cphn2; CyP 20b; CYP S1; CYP-S1; CYPB; MGC14109; MGC2224; peptidyl prolyl cis trans isomerase B; Peptidyl prolyl cis trans isomerase B precursor; Peptidyl-prolyl cis-trans isomerase B precursor; Peptidylprolyl isomerase B; PPIase; PPIB; Rotamase; S cyclophilin; SCYLP; PPIB_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclophilin B (Loading Control)) Monoclonal Antibody, Unconjugated (orb704516) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclophilin B (Loading Control)) Monoclonal Antibody, Unconjugated (orb704516) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: Thyroid gland (Rat) Lysate at 40 ug, Lane 5: Thyroid gland (Mouse) Lysate at 40 ug, Lane 6: Placenta (Rat) Lysate at 40 ug, Lane 7: Placenta (Mouse) Lysate at 40 ug, Lane 8: Cerebrum (Rat) Lysate at 40 ug, Lane 9: Cerebrum (Mouse) Lysate at 40 ug, Lane 10: Liver (Rat) Lysate at 40 ug, Lane 11: Liver (Mouse) Lysate at 40 ug, Lane 12: Kidney (Rat) Lysate at 40 ug, Lane 13: Kidney (Mouse) Lysate at 40 ug, Lane 14: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-Cyclophilin B (orb704516) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 19 kD, Observed band size: 19 kD.

Sample: Lane 1: Placenta (Rat) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Liver (Rat) Lysate at 40 ug, Lane 6: Kidney (Rat) Lysate at 40 ug, Lane 7: Hela (Human) Cell Lysate at 30 ug, Lane 8: A431 (Human) Cell Lysate at 30 ug, Lane 9: HepG2 (Human) Cell Lysate at 30 ug, Lane 10: Huvec (Human) Cell Lysate at 30 ug, Lane 11: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-Cyclophilin B (orb704516) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 18 kD.

Quick Database Links

Gene Symbol

PPIB

UniProt

P23284