Cycloleucine

SKU: orb1940166

Description

Cycloleucine is an antagonist of NMDA receptor associated glycine receptor with a Ki of 600 μM. Cycloleucine is also a competitive inhibitor of S-adenosyl-methionine mediated methylation with anxiolytic and cytostatic effects.(In Vitro):Cytostatic (10 μg/mL) inhibits the viability of human KB and mouse L1210s leukemia cell lines. Cycloleucine (4-40 mM; 3 h) blocks internal methylation of viral RNA in B77 transformed chick embryo fibroblasts. Cycloleucine (40 mM; 24 h) blocks the formation of both m6A and the penultimate Gm in B77 38S RNA subunits by greater than 90%.(In Vivo):Cycloleucine (0.5, 1.0, 2.0, 4 μg/μL; intracerebroventrically) increases time spent in open arms, open arm entries, and extreme arrivals in rats. In Semliki Forest virus strain A7(74) infected and control mice, Cycloleucine reduces thymus and spleen weights.

Key Properties

• CAS Number: 52-52-8
• MW: 129.159
• Purity: >98% (HPLC)
• Formula: C₆H₁₁NO₂
• SMILES: NC1(CCCC1)C(O)=O
• Target: NMDAR
• Solubility: In Vitro: H2O : 20 mg/mL (154.85 mM)

Bioactivity

• In Vivo:
  Cycloleucine (0.5-4 μg; intracerebroventrical injection) increases time spent in open arms, open arm entries, and extreme arrivals in rats. Cycloleucine reduces thymus and spleen weights in Semliki Forest virus (SFV) strain A7(74) infected and control mice. Animal model: Male rats bilaterally cannulated into the nucleus accumbens septi (NAS). Dosage: 1 μL of 0.5, 1.0, 2.0, 4 μg/μL. Administration: Intracerebroventrical injection. Result: Increased time spent in the open arms and extreme arrivals at all doses. Increased open arm entries at the dose of 4 μg.
• In Vitro:
  Cycloleucine (4-40 mM; 3 h) blocks internal methylation of viral RNA in B77 transformed chick embryo fibroblasts. Cycloleucine (40 mM; 24 h) blocks the formation of both m6A and the penultimate Gm in B77 38S RNA subunits by greater than 90%. Cytostatic (10 μg/mL) inhibits the viability human KB and mouse L1210s leukemia cell lines.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

NMDAR