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Cyclin E1 (phospho Thr77) rabbit pAb

Catalog Number: orb770794

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb770794
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Cyclin E1 (phospho-Thr77)
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr77. AA range:43-92
Concentration1 mg/ml
Dilution rangeImmunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
ConjugationUnconjugated
MW47kD
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-CCNE1 antibody, anti-CCNE antibody, anti-G1/S
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NoteFor research use only
Expiration Date12 months from date of receipt.
Cyclin E1 (phospho Thr77) rabbit pAb

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin E1 (Phospho-Thr77) Antibody.

Cyclin E1 (phospho Thr77) rabbit pAb

Immunofluorescence analysis of HeLa cells, using Cyclin E1 (Phospho-Thr77) Antibody. The picture on the right is blocked with the phospho peptide.

Cyclin E1 (phospho Thr77) rabbit pAb

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).