You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb770794 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Cyclin E1 (phospho-Thr77) |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr77. AA range:43-92 |
Concentration | 1 mg/ml |
Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 47kD |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-CCNE1 antibody, anti-CCNE antibody, anti-G1/S Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin E1 (Phospho-Thr77) Antibody.
Immunofluorescence analysis of HeLa cells, using Cyclin E1 (Phospho-Thr77) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).