Cyclin E1 (phospho Thr395) rabbit pAb

• Catalog Number: orb764170
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cyclin E1 (phospho-Thr395)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr395. AA range:361-410
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 48
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CCNE1 antibody, anti-CCNE antibody, anti-G1/S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of HeLa cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1:2000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin E1 (Phospho-Thr395) Antibody.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Cyclin E1 (Phospho-Thr395) Antibody. The picture on the right is blocked with the phospho peptide.