Cyclin E1 Antibody / CCNE1

• Catalog Number: orb1151689
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cyclin E1
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rCCNE1/4936
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 10-176 was used as the immunogen for the Cyclin E1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Cyclin E1 antibody is available for research use only.
• UniProt ID: P24864
• Storage: Aliquot the recombinant Cyclin E1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Cyclin E1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HCT116 cell lysate using recombinant Cyclin E1 antibody (clone rCCNE1/4936). Predicted molecular weight ~47 kDa.

IHC staining of FFPE human ovarian carcinoma tissue with recombinant Cyclin E antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with recombinant Cyclin E1 antibody (clone rCCNE1/4936). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Cyclin E1 antibody (clone rCCNE1/4936) as confirmation of integrity and purity.