Cyclin E1 Antibody / CCNE1

• Catalog Number: orb1151663
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Cyclin E1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 10-176 was used as the immunogen for the recombinant CCNE1 antibody.
• UniProt ID: P24864
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant CCNE1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CCNE1/4935R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant CCNE1 antibody is available for research use only.
• Note: For research use only

Western blot testing of human HCT-116 cell lysate using recombinant CCNE1 antibody (clone CCNE1/4935R). Predicted molecular weight ~47 kDa.

IHC staining of FFPE human endometrium with recombinant CCNE1 antibody (clone CCNE1/4935R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian carcinoma tissue with recombinant CCNE1 antibody (clone CCNE1/4935R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CCNE1 antibody (clone CCNE1/4935R) as confirmation of integrity and purity.