Ccnd3 Antibody

SKU: orb420319

Description

Cyclin D3 antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, IP, WB
• Dilution Range: ELISA: 1:1000-1:5000, FC: 4µg/mL, IHC: 4µg/mL, IP: 5µg/mL, WB: 1.0 ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, flow cytometry, and by immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 32.4 kDa in size corresponding to Cyclin D3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This reagent reacts equally to phosphorylated and non-phosphorylated Cyclin D3 by ELISA and western blotting.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
• Target: Ccnd3
• Purity: Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Cyclin D3.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Cyclin D3 antibody, G1/S-specific cyclin-D3, Ccnd3, Cyl-3

ELISA results of purified Rabbit Anti-Cyclin D3 Antibody tested against BSA-conjugated peptide of cyclin D3 peptide. Each well was coated in duplicate with 0.1 µg of conjugate (red line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) and TMB substrate (p/n orb348651).

Flow Cytometry of Rabbit anti-Cyclin D3 antibody. Cells: mouse B cells +, 14 days post immunization (NP-CGG). Anti-Cyclin D3-RPE antibody at 1:500 for 20 min at 4°C.

Immunohistochemistry with anti-Cyclin D3 antibody showing Cyclin D3 staining in nucleus of lymphocytes in human spleen at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH6.2 and then incubated with rabbit anti-mouse Cyclin D3 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.

Immunoprecipitation of Rabbit Anti-Cyclin D3. Lane 1: 5 µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D3 Mouse mAb. Lane 2: 5 µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D2 rabbit pAB. Secondary Antibody: TrueBlot IgG IP beads. Expect: ~32kDa to D3 in lane 1. No reactivity to D2 in lane 2.

Quick Database Links

Gene Symbol

Ccnd3

UniProt

P30282