Cyclin D1 Rabbit mAb [KD Validated] Antibody

SKU: orb1951725

Description

Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:100 IP 1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Cyclin D1
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCND1;BCL1; D11S287E; PRAD1; U21B31; Cyclin D1;

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Immunofluorescent analysis of MCF-7 cells, using Cyclin D1 Antibody.

Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody.

Western blot analysis of Cyclin D1 expression in (1) MCF-7 cell lysates; (2) LnCaP cell lysates.

Quick Database Links

UniProt

P24385