Cyclin D1 Antibody / CCND1

• Catalog Number: orb699718
• Category: Antibodies
• Description: Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 270-295 fro the human protein was used as the immunogen for the recombinant Cyclin D1 antibody.
• UniProt ID: P24385
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the recombinant Cyclin D1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CCND1/3370R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free recombinant Cyclin D1 antibody (clone CCND1/3370R) as confirmation of integrity and purity.

IHC staining of FFPE human mantle cell carcinoma with recombinant Cyclin D1 antibody (clone CCND1/3370R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human mantle cell carcinoma with recombinant Cyclin D1 antibody (clone CCND1/3370R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.