Cyclin B1 Rabbit mAb [KD Validated]

• Catalog Number: orb1951656
• Category: Antibodies
• Description: Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF).
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Cyclin B1
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:100
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P14635
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CCN-2; CCNB; CCNB1; CCNB1-RS1; CYCB; CYCB1; G2/mit
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells, using Cyclin B1 Antibody.

Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody.

Western blot analysis of Cyclin B1 expression in HeLa cell lysate.