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Catalog Number | orb1264224 |
---|---|
Category | Antibodies |
Description | CYC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of human CYC1. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 35 kDa |
Target | CYC1 |
UniProt ID | P08574 |
NCBI | P08574 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Cytochrome c1, heme protein, mitochondrial, Comple Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis in Hela, MCF-7 cell line lysates (35 ug/lane).
CYC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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