CXCR3 Antibody

SKU: orb1247639

Description

CXCR3 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments showed a band at approx 55-60kDa in Human Tonsil and Mouse Spleen lysates after 1ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note we cannot currently find an explanation in the literature for this band, given the calculated size of 45.5kDa according to NP_001136269.1Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RRDSSWSETSEA
• Target: CXCR3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

CXCR3, chemokine (C-X-C motif) receptor 3, HGNC:4540, CD183, CKR-L2, CMKAR3, GPR9, IP10, IP10-R, Mig-R, MigR, G protein-coupled receptor 9, IP10 receptor, Mig receptor, chemokine (C-X-C) receptor 3

orb1247639 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI.

orb1247639 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI.

orb1247639 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

CXCR3

UniProt

P49682

RefSeq (Protein):NP_001136269.1, NP_001495.1