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| Catalog Number | orb1538959 |
|---|---|
| Category | Antibodies |
| Description | CXCL7/PPBP Antibody |
| Target | CXCL7 / PPBP |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2 |
| Purification | Immunoaffinity purified |
| Immunogen | E.coli derived recombinant Human NAP-2. |
| Tested applications | ELISA, IHC, IHC-P, WB |
| Dilution range | ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) |
| Application notes | Resuspension: Reconstitute with sterile water. Further information: Immunohistochemistry: LS-B7394 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hNAP-2 (100 ng/ml), a concentration of 5-10 ug/ml of this antibody is required. ELISA: To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NAP-2 (LS-C104604) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | PPBP, Beta-thromboglobulin, B-TG1, Beta-TG, C-X-C Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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