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CXCL16 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCXCL16 (human CXCL16). |
| Target | CXCL16 |
| Purification | Anti-hCXCL16 specific antibody was purified by affinity chromatography employing immobilized hCXCL16 matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
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48 T, 96 TRat Chemokine C-X-C-Motif Ligand 16 (CXCL16) ELISA Kit [orb775570]
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96 T, 48 TCXCL16 Rabbit Polyclonal Antibody [orb11355]
FC, IF, IHC-Fr, IHC-P
Rat
Human, Mouse
Rabbit
Polyclonal
Unconjugated
100 μl, 200 μl, 50 μlCXCL16 Rabbit Polyclonal Antibody (FITC) [orb559554]
FC, IF
Rat
Human, Mouse
Rabbit
Polyclonal
FITC
100 μl

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To detect hCXCL16 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human CXCL16 (orb1272574) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCXCL16.

To detect hCXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed paraffin-embedded sections of human colorectal adenocarcinoma tissue. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen retrieval is not required for these conditions. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed paraffin-embedded sections of human colorectal adenocarcinoma tissue. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen retrieval is not required for these conditions. Optimal concentrations and conditions may vary.
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Protocol Information
CXCL16 Antibody (orb1272575)
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