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Catalog Number | orb1539119 |
---|---|
Category | Antibodies |
Description | CXCL11 Antibody |
Target | CXCL11 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2 |
Immunogen | E.coli derived recombinant Human I-TAC. |
Tested applications | ELISA, IHC, IHC-P, WB |
Dilution range | ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) |
Application notes | Resuspension: Reconstitute with sterile water. Further information: Immunohistochemistry: LS-B8212 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hI-TAC (100 ng/ml), a concentration of 7-8 ug/ml of this antibody is required. ELISA: To detect hI-TAC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human I-TAC (LS-C104528) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hI-TAC. Western Blot: To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3 ng/lane, under either reducing or non-reducing conditions. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CXCL11, Beta-R1, Chemokine h174, Chemokine ip-9, C Read more... |
Note | For research use only |
ELISA, ICC, IF, IHC-P, WB | |
Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |