CVT-313

SKU: orb1304438

Description

CVT-313 (NG-26) is a potent and selective research compound that acts as a reversible, ATP-competitive inhibitor. It is utilized in biochemical and cellular studies to investigate kinase function in pathways relevant to cancer and cell cycle regulation, with applications in both in vitro and in vivo experimental models.

Research Area

Cell Biology

Key Properties

• CAS Number: 199986-75-9
• MW: 400.47
• Purity: 97.46% (May vary between batches)
• Formula: C₂₀H₂₈N₆O₃
• SMILES: COc1ccc(CNc2nc(nc3n(cnc23)C(C)C)N(CCO)CCO)cc1
• Target: CDK
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.99 mM);DMSO:50 mg/mL (124.85 mM)

Bioactivity

• Target IC50:
  CDK2:0.5 μM|Cdk4-CyclinD1:215 μM|CDK1-CyclinB:4.2 μM
• In Vitro:
  CVT-313 has been shown to inhibit other kinases, but at much higher IC50 values, i.e., CDK1 (IC50=4.2 μM), CDK4 D1 (IC50=215 μM), and MAPK/PKA/PKC (IC50>1.25 mM), compared to CDK2 (IC50=0.5 μM). CVT-313 has been shown to have profound effects on cell proliferation at concentrations of 5-20 μM. CVT-313 is a potent CDK2 inhibitor, which is identified from a purine analog library with an IC50 of 0.5 μM in vitro. Inhibition is competitive with respect to ATP (Ki=95 nM), and selective CVT-313 has no effect on other, nonrelated ATP-dependent serine/threonine kinases. When added to CDK1 or CDK4, a 8.5- and 430-fold higher concentration of CVT-313 is required for half-maximal inhibition of the enzyme activity. Using normal and tumor human/murine cell lines, the effects of CVT-313 on cell proliferation is measured. The IC50 for growth inhibition ranged from 1.25 to 20 μM.
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• Cell Research:
  CVT313 is prepared in DMSO and stored, and then diluted with appropriate medium before use. MRC-5 cells are grown in Dulbecco's modified Eagle's medium containing 5% fetal calf serum. CVT313 (0, 5, 10, 15 μM) is added to exponentially growing cells in tissue culture. Cell population is measured. Proliferation assays are carried out using the nonradioactive CellTiter 96 kit after 48-h exposure. For FACS analysis of DNA content, cells are trypsinized, fixed in 70% ice-cold ethanol, and treated with 0.1 mg/mL RNase A and 40 μg/mL propidium iodide for 1 h at 37°C.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDK, CDK4, CDK1, Cdk2 Inhibitor III, CDK2, CVT313, CVT-313, Cyclin dependent kinase, CVT 313, Inhibitor, inhibit, NG 26, NG26, NG-26

Compound Identifiers

PubChem CID

6918386