CUL2 Antibody

SKU: orb1249102

Description

CUL2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 87.0kDa according to Human NP_003582.2 and of 86.9kDa according to Mouse NP_083678.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QYIERSQASADE
• Target: CUL2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

CUL2, cullin 2, MGC131970, OTTHUMP00000019437

orb1249102 (1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249102 (3.75 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CUL2

UniProt

Q13617

RefSeq (Protein):NP_003582.2