CUL1 Antibody

SKU: orb750497

Description

CUL1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000
• Reactivity: Human
• Application Notes:
  Anti-Cul1 has been tested by immunohistochemistry. This antibody reacts with human Cul1 by immunohistochemistry, western blot, and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, but rather use 0.1% SDS. An 89.6 kDa band corresponding to human Cul1 is detected. Most cell lines expressing Cul1 can be used as a positive control. Researchers should determine optimal titers for other applications.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal near amino acids 750-776 of Human Cul1 coupled to KLH.
• Target: CUL1
• Purity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Cul1 Antibody, rabbit anti-Cullin1 antibody, MGC149834 antibody, MGC149835 antibody, CUL, Cullin

Quick Database Links

Gene Symbol

CUL1

UniProt

Q13616

RefSeq:32307161