CUL1 Antibody

SKU: orb750497

Description

CUL1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000
• Reactivity: Human
• Application Notes:
  Anti-Cul1 has been tested by immunohistochemistry. This antibody reacts with human Cul1 by immunohistochemistry, western blot, and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, but rather use 0.1% SDS. An 89.6 kDa band corresponding to human Cul1 is detected. Most cell lines expressing Cul1 can be used as a positive control. Researchers should determine optimal titers for other applications.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal near amino acids 750-776 of Human Cul1 coupled to KLH.
• Target: CUL1
• Purity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Cul1 Antibody, rabbit anti-Cullin1 antibody, MGC149834 antibody, MGC149835 antibody, CUL, Cullin

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF (SKP1/cullin-1/F-box protein) / CBC (cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.

Biorbyt's Anti-CUL1 antibody was diluted 1:500 to detect CUL1 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Western Blot of Anti-Cul1 Antibody. Conformational control of CRL activities. (A) Polyubiquitination reactions with SCFβTRCP/β-catenin phosphopeptide (left), and SCFSkp2/CksHs1/ phospho-p27 (right) reconstituted with non-NEDD8ylatable (K720R), un-NEDD8ylated wild-type (WT), and fully NEDD8ylated Cul1-Rbx1 (WT~N8). Reaction products were detected by immunoblotting, top panels with anti-biotin (left) or anti-p27 (right), middle with anti-His (Ubiquitin; green * - Cul1~Ubiquitin), and lower with anti-Cul1 C-terminus antisera.

Western Blot of Anti-Cul1 Antibody. Functional analysis of mutations influencing RING conformational flexibility. B, NEDD8ylation time-course for wild-type (wt) Cul1-Rbx1, non-NEDD8ylatable (K720R) control, and variants harboring the indicated Rbx1 linker Val insertion/deletion or Pro mutations, detected by western blotting with anti-Cul1 C-terminus antisera.

Quick Database Links

Gene Symbol

CUL1

UniProt

Q13616

RefSeq:32307161