Cucurbitacin B

SKU: orb1223089

Description

Cucurbitacin B is a plant-derived triterpene that has the classic four-ring structure of mammalian steroids. Cucurbitacin B suppresses metastasis mediated by reactive oxygen species (ROS) via focal adhesion kinase (FAK) in breast cancer MDA-MB-231 cells. Cucurbitacin B induces inhibitory effects via CIP2A/PP2A/Akt pathway in glioblastoma multiforme. Cucurbitacin B acts a potential insect growth regulator by antagonizing 20-hydroxyecdysone activity.

Key Properties

• CAS Number: 6199-67-3
• MW: 558.7
• Purity: >98% (HPLC)
• Formula: C₃₂H₄₆O₈
• SMILES: C(=O)(C)OC(C)(/C=C/C(=O)[C@](C)(O)[C@H]1[C@@H](C[C@]2([C@@H]3CC=C4C(C(=O)[C@H](C[C@H]4[C@@]3(C(=O)C[C@]12C)C)O)(C)C)C)O)C
• Target: Hedgehog (Hh)
• Solubility: DMSO : ≥ 100 mg/mL 178.99 mM; H2O : < 0.1 mg/mL

Bioactivity

• In Vivo:
  Animal model: Carrageenan-induced prostatic inflammation in rats. Dosage: 5 mg/kg/day for 10day. Administration: Oral administration. Result: Decreased the level of TNF-α, IL-1b, COX-2 and iNOS in prostatic tissues. Animal model: STZ-ICV rat prototype of AD-like dementia. Dosage: 20, 50 mg/kg/day for 28days. Administration: Intraperitoneal injection (i.p.). Result: Decreased the TNF-α, IL-1β, MPO, iNOS, acetylcholinesterase, and glutamate levels, but increased gamma-aminobutyric acid. Increase in viable neuron density in the cortex and hippocampus of rats.
• In Vitro:
  Cell Viability Assay Cell line: CCA cell lines. Concentration: 0.1, 1 0.5, 1, 5, 10, 20, 40 μM Incubation time: 24 and 48 h. Result: Decreased cell viability in a dose-dependent and time-dependent manner with the IC50 values of 13: 44 μM for 24 h and 1.55 for 48 h. Cell Cycle Analysis Cell line: CCA cell lines. Concentration: 0.1, 1, 10 μM Incubation time: 24 h. Result: Arrested cell cycle progression at the G2/M phase. Western blot analysis. Cell line: CCA cell lines. Concentration: 0.1, 1 0.5, 1, 5, 10, 20, 40 μM Incubation time: 12 and 24 h. Result: Decreased the expression of Cyclin A, Cyclin D1, Cdc25A, but increased the level of p21.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cucurbitacin B | Cuc B | NSC 49451

Quick Database Links

Gene Symbol

Hedgehog (Hh)