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Item 1 of 5
CTSE Antibody (Center)
Catalog Number: orb1929981
| Catalog Number | orb1929981 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19246 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1：500-1：2000, WB: 1:2000, WB: 1:2000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 42794 Da |
| Target | This CTSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-187 amino acids from the Central region of human CTSE. |
| UniProt ID | P14091 |
| NCBI | NP_683865.1, NP_001901.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Cathepsin E, Cathepsin E form I, Cathepsin E form Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
CTSE Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CTSE Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTSE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-CTSE Antibody (Center) at 1:2000 dilution. Lane 1: MGC803 whole cell lysate. Lane 2: MKN45 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CTSE Antibody (Center) at 1:500-1:2000 dilution. Lane 1: Mouse spleen whole tissue lysate. Lane 2: MKN45 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CTSE Antibody (Center) at 1:2000 dilution. Lane 1: Mouse stomach whole tissue lysate. Lane 2: Rat stomach whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
