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CTSD/Cathepsin D Antibody

SKU: orb1538710

Description

CTSD/Cathepsin D Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, IP, WB
Dilution RangeELISA, IHC, IHC-P (5 µg/ml), IP, WB (1:500 - 1:1000)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1,k
Clone No.3F12-1B9
ImmunogenCTSD (AAH16320, 26 aa ~ 412 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
TargetCTSD / Cathepsin D
PurificationPurified from ascites by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, pH 7.4
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CTSD, CPSD, CLN10, Lysosomal aspartyl peptidase, Lysosomal aspartyl protease, ProCathepsin D, CatD, Cathepsin D

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CTSD/Cathepsin D Antibody

Western blot of CTSD expression in transfected 293T cell line by CTSD antibody. Lane 1: CTSD transfected lysate (Predicted MW: 44.6 KDa). Lane 2: Non-transfected lysate.

CTSD/Cathepsin D Antibody

Immunoprecipitation of CTSD transfected lysate using anti-CTSD monoclonal antibody and Protein A Magnetic Bead.

CTSD/Cathepsin D Antibody

Detection limit for recombinant GST tagged CTSD is approximately 3 ng/ml as a capture antibody.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

CTSD/Cathepsin D Antibody (orb1538710)

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50 μg
$ 640.00
DispatchUsually dispatched within 2-3 weeks
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