Cathepsin D Antibody / CTSD

SKU: orb2643060

Description

Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 52kDa preprocathepsin D that is cleaved and glycosylated to form a 48kDa procathepsin D and then further cleaved to produce 34kDa and 14kDa subunits (heavy and light chains, respectively). Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 0.5-1ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CTSD antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CTPD-1
• Immunogen: Amino acids 104-250 were used as the immunogen of the Cathepsin D antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human liver tissue with Cathepsin D antibody (clone CTPD-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human liver lysate with CTSD antibody (clone CTPD-1).

Quick Database Links

UniProt

P07339