CTLA4 Antibody (N-term)

SKU: orb1938971

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:50-100
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB23352
• Immunogen: This IDDM12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids of human IDDM12. Antigen Region: 50-78 aa.
• Target: CTLA4
• Molecular Weight: 24656 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4, CD152, IDDM12

All lanes: Anti- CTLA4 Antibody (N-term) at 1:2000 dilution. Lane 1: Raji whole cell lysate. Lane 2: U266B1 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: CCRF-CEM whole cell lysate. Lane 6: Human spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti- CTLA4 Antibody (N-term) at 1:2000 dilution. Lane 1: WiDr whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: CCRF-CEM whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CTLA4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTLA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

CTLA4

UniProt

P16410

RefSeq (Protein):NP_005205.2, NP_001032720.1