CTBP1 Antibody (C-term)

SKU: orb1788395

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, IF: 1:10~50, WB: 1:1000, WB: 1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This CTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human CTBP1. Antigen Region: 413-440 aa.
• Target: CTBP1
• Molecular Weight: 47535 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CTBP1; CTBP; C-terminal-binding protein 1

Western blot analysis of lysates from Jurkat cell line, mouse brain, rat brain tissue lysate, Raji, Hela cell line (from left to right), using CTBP1 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

All lanes: Anti-CTBP1 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Raji whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of Hela cell stained with CTBP1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.

Fluorescent confocal image of SK-BR-3 cell stained with CTBP1 Antibody (C-term). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.

Quick Database Links

Gene Symbol

CTBP1

UniProt

Q13363

RefSeq (Protein):NP_001319.1, NP_001012632.1