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Catalog Number | orb1257385 |
---|---|
Category | Antibodies |
Description | CSTA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTA (NP_005204.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 13kDa |
Target | CSTA |
UniProt ID | P01040 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Cystatin-A, Cystatin-AS, Stefin-A, Cystatin-A, N-t Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of THP-1 cells, using CSTA antibody (orb1257385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using CSTA antibody (orb1257385). Blue: DAPI for nuclear staining.
IF, IH, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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