CSRP3 Antibody

SKU: orb1246610

Description

CSRP3 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 20kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 21.0kDa according to Human NP_003467.1 and 20.8kDa according to Rat NP_476485.1). Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 2.5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CGKSLESTNVTDKD
• Target: CSRP3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CSRP3, cysteine and glycine-rich protein 3 (cardiac LIM protein), CLP, CMD1M, CRP3, LMO4, MGC14488, MGC61993, MLP, LIM domain only 4, cardiac LIM domain protein, cardiac LIM protein, cysteine and glycine-rich protein 3, cysteine-rich protein 3, muscle LIM protein

orb1246610 (0.01 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246610 (2.5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246610 (2.5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CSRP3

UniProt

P50461

RefSeq (Protein):NP_003467.1