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CSNK2B Antibody

SKU: orb1248050

Description

CSNK2B Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman, Mouse, Porcine, Rat
Predicted ReactivityBovine, Canine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in lysates of cell lines NIH3T3, HeLa, K562, MCF7 and HepG2 (calculated MW of 24.9kDa according to NP_001311.3). Approx 25kDa band observed in Mouse Testis, 26kDa in Rat Testis and 28kDa in Pig Testis lysates (calculated MW of 24.9kDa according to Mouse NP_001290374.1, Rat NP_001030315.1 and pig NP_001138849.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-TGLNEQVPHYRQ
TargetCSNK2B
Molecular WeightApprox 26kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CSNK2B, casein kinase 2, beta polypeptide, CK2B, CK2N, CSK2B, G5A, CK II beta, Casein kinase II beta subunit, alternative name: G5a, phosvitin, casein kinase II subunit beta, phosvitin, protein G5a

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CSNK2B Antibody

orb1248050 (1 ug/ml) staining of NIH3T3 (A), HeLa (B), K562 (C), MCF7 (D) and HepG2 (E) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CSNK2B Antibody

orb1248050 (0.3 ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Testis lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CSNK2B Antibody (orb1248050)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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