CSNK1E Antibody, KO Validated

• Catalog Number: orb1274480
• Category: Antibodies
• Description: CSNK1E Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 47kDa
• Target: CSNK1E
• UniProt ID: P49674
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CSNK1E antibody (orb1274480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody (orb1274480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (orb1274480) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (orb1274480) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using CSNK1E antibody (orb1274480). Blue: DAPI for nuclear staining.