CSDE1 Antibody

• Catalog Number: orb1257243
• Category: Antibodies
• Description: CSDE1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 102kDa
• Target: CSDE1
• UniProt ID: O75534
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Cold shock domain-containing protein E1, N-ras ups
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CSDE1 antibody (orb1257243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunoprecipitation analysis of 100 ug extracts of HeLa cells using 3 ug CSDE1 antibody (orb1257243). Western blot was performed from the immunoprecipitate using CSDE1 antibody (orb1257243) at a dilution of 1:1000.