NLRP3 Antibody

SKU: orb1247643

Description

NLRP3 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:This antibody is not recommended for use in Western blotting.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of human peripheral blood monocyte cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EKPELTVVFEPSW
• Target: NLRP3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NLRP3, CIAS1, FCU, MWS, FCAS, NALP3, C1orf7, PYPAF1, AII/AVP, AGTAVPRL, CRYOPYRIN, cold autoinflammatory syndrome 1, cryopyrin, Muckle-Wells syndrome, chromosome 1 open reading frame 7, PYRIN-containing APAF1-like protein 1, neuronal apoptosis inhibitor protein 3, angiotensin/vasopressin receptor AII/AVP-like, NLR family, pyrin domain containing 3, AII, AVP, CLR1.1, FLJ95925, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3

orb1247643 (5 ug/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247643 Immunofluorescence analysis of paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247643 Flow cytometric analysis of paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goa

Quick Database Links

Gene Symbol

NLRP3

UniProt

Q96P20

RefSeq (Protein):NP_899632.1, NP_004886.3, NP_001073289.1, NP_001120934.1, NP_001120933.1