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Catalog Number | orb1257820 |
---|---|
Category | Antibodies |
Description | CRYM Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human CRYM (NP_001879.1). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 36kDa |
Target | CRYM |
UniProt ID | Q14894 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CRYM, Crystallin, mu, Ketimine reductase, Mu-cryst Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CRYM antibody (orb1257820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts of rat brain, using CRYM antibody (orb1257820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CRYM antibody (orb1257820) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CRYM antibody (orb1257820) at dilution of 1:100 (40x lens).
ELISA, IHC-P, IP, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |