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Catalog Number | orb1930234 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB1846 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 66947 Da |
Target | This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Cry2. |
UniProt ID | Q49AN0 |
NCBI | NP_001120929.1, NP_066940.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Cryptochrome-2, CRY2, KIAA0658 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of hCry2-R579 in mouse liver tissue and HepG2 cell line lysates (35 ug/lane). CRY2 (arrow) was detected using the purified Pab.
Western blot analysis of lysates from human skeletal muscle and human heart tissue (from left to right), using Cry2 Antibody (R579). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
Western blot analysis of lysates from human heart, skeletal muscle and liver tissue lysate (from left to right), using Cry2 Antibody (R579). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.