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Catalog Number | orb1257699 |
---|---|
Category | Antibodies |
Description | CRTAP Antibody |
Target | CRTAP |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human CRTAP (NP_006362.1). |
UniProt ID | O75718 |
MW | Observed: 47kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Cartilage-associated protein, CRTAP, CASP |
Note | For research use only |
Western blot analysis of extracts of SW480 cells, using CRTAP antibody (orb1257699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using CRTAP antibody (orb1257699) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using CRTAP antibody (orb1257699) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CRTAP antibody (orb1257699) at dilution of 1:100 (40x lens).
ELISA, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |