CSRP2 Antibody

SKU: orb1247645

Description

CSRP2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Lung shows staining of the endothelial cells of a blood vessel. Recommended concentration: 3-6ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ERLGIKPESVQP
• Target: CSRP2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

CRP2, CSRP2, cysteine and glycine-rich protein 2, LMO5, SmLIM, LIM domain only 5, smooth muscle

orb1247645 (3.8 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

HEK293 overexpressing CRP2 and probed with orb1247645 (mock transfection in first lane).

orb1247645 (0.2 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence

Quick Database Links

Gene Symbol

CSRP2

UniProt

Q16527

RefSeq (Protein):NP_001312.1