Crotonyl-HIST1H4A (K5) Antibody

• Catalog Number: orb418605
• Category: Antibodies
• Description: This Crotonyl-HIST1H4A (K5) Antibody is an unconjugated polyclonal product. It tagets HIST1H4A using a Peptide sequence around site of Crotonyl-Lys (5) derived from Human Histone H4 as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IP, WB. Purification: Antigen Affinity Purified.
• Target: HIST1H4A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of Crotonyl-Lys (5) derived from Human Histone H4
• UniProt ID: P62805
• Tested applications: ChIP, ELISA, IF, IP, WB
• Dilution range: WB:1:500-5000, IF:1:50-200, IP:1:200-2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Histone H4 antibody, anti- HIST1H4A antibody,
• Research Area: Epigenetics & Chromatin
• Note: For research use only

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Crotonyl-HIST1H4A (K5) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysateusing Crotonyl-HIST1H4A (K5) antibody

Immunoprecipitation analysis of Hela whole cell lysate using Crotonyl-HIST1H4A (K5) antibody

Immunofluorescence staining of Hela cells using Crotonyl-HIST1H4A (K5) antibody