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| Catalog Number | orb764323 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Crk II (phospho-Tyr221) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Monkey, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. AA range:187-236 |
| MW | 40 |
| Tested applications | ELISA, IF, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-CRK antibody, anti-Adapter molecule crk antib Read more... |
| Note | For research use only |
Western Blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CrkII (Phospho-Tyr221) Antibody.
Immunofluorescence analysis of HUVEC cells, using CrkII (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COS7 cells, using CrkII (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide.
