Cripto-1 TDGF1 antibody

• Catalog Number: orb345584
• Category: Antibodies
• Description: Cripto-1 TDGF1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.
• Concentration: 0.97 mg/mL
• Dilution range: ELISA: 1:105,000, IHC: User Optimized, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human Cripto-1 protein. A BLAST analysis was used to suggest limited cross-reactivity with Cripto-1 from mouse based on a 70% homology with the immunizing sequence. Expect cross-reactivity with human Cripto-3 (TDGF2) based on very high levels of sequence conservation. Cross-reactivity with Cripto-1 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P13385
• NCBI: 4507425
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Cripto 1 antibody, rabbit anti-Cripto-
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to Cripto-1 by western blotting in the appropriate cell lysate or extract. Cripto-1 is reported as a 188 amino acid protein with a molecular weight of 21.1 kDa.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells using Cripto-1 TDGF1 antibody