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Catalog Number | orb1256927 |
---|---|
Category | Antibodies |
Description | CRHBP Antibody |
Target | CRHBP |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2). |
UniProt ID | P24387 |
MW | Observed: 36kDa |
Tested applications | IF, IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CRF-BP, CRFBP, corticotropin-releasing factor-bind Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using CRHBP antibody (orb1256927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using CRHBP antibody (orb1256927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CRHBP antibody (orb1256927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CRHBP antibody (orb1256927) at dilution of 1:100 (40x lens).
WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
HRP |
Rat | |
15.63-1000 pg/mL | |
6.6 pg/mL |