CRH Antibody

SKU: orb1239958

Description

CRH Antibody

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH.The immunogen is located within amino acids 30 - 80 of CRH.
• Target: CRH
• Molecular Weight: Predicted: 22 kDa Observed: 29 kDa
• Purification: CRH antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CRH antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRH Antibody: CRF, Corticoliberin, Corticotropin-releasing factor, CRF

Western blot analysis of CRH in human placenta tissue lysate with CRH antibody at (A) 1 and (B) 2 µg/ml.

Immunohistochemistry of CRH in human brain tissue with CRH antibody at 5 µg/mL.

Immunofluorescence of CRH in human brain tissue with CRH antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

CRH

UniProt

P06850

RefSeq (Protein):NP_000747