CRF-RII rabbit pAb

SKU: orb767692

Description

Rabbit polyclonal antibody to CRF-RII

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CRHR2. AA range:71-120
• Molecular Weight: 48
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CRHR2 antibody, anti-CRF2R antibody, anti-CRH2R antibody, anti-Corticotropin-releasing factor receptor 2 antibody, anti-CRF-R-2 antibody, anti-CRF-R2 antibody, anti-CRFR-2 antibody, anti-Corticotropin-releasing hormone receptor 2 antibody, anti-CRH-R-2 antibody, anti-CRH-R2 antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of MCF7 cells, using CRHR2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 and RAW264.7 cells, using CRHR2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using CRHR2 antibody.